amplify provides a suite of functions to analyze routine ΔΔCt qPCR data away from the QuantStudio, with greater flexibility, by providing features such as:

• tidying data from off the QuantStudio
• Plate and expression plotting
• (Re)calculation of RQ
• Resetting of control probe

For more information, see Analyzing ddCt qPCR with amplify

Additionally, amplify automatically generates QC reports for routine standard-curve library-preparation quantification assays (see Performing Library Quantification QC).

plan-pcr

amplify is used by plan-pcr (source/README), a Shiny app used to help make plate layouts, calculate dilutions and amounts of mastermix needed, and generate reports.

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